The enzyme-linked immunosorbent spot (ELISpot) is a type of assay that focuses on quantitatively measuring the frequency of cytokine secretion for a single cell.

Jul 3, 2025 · ELISPOT is a highly sensitive assay designed to detect secreted proteins from individual cells, making it ideal for immunological studies. Developed as an extension of ELISA, ELISPOT …

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

May 23, 2022 · ELISpot has evolved from a research tool to an important clinical assay. Recent studies of cancer vaccines tested in a variety of malignancies have suggested that ELIspot may be a useful …

Nov 10, 2022 · ELISpot is an immunoassay used to quantify analyte-secreting cells. Cytokines, immunoglobulins, or other target proteins secreted by cells are captured by specific antibodies …

Jul 27, 2025 · ELISPOT, or Enzyme-Linked ImmunoSpot, is a laboratory technique used to measure immune responses. It directly quantifies specific immune cells actively producing certain molecules.

ELISPOT is the most sensitive technique available for the detection of immune cells which secrete specific signature proteins such as antibodies or cytokines. It is the state-of-the-art method for the …

The cytokine ELISpot assay has been widely applied to investigate specific immune responses in infections, cancer, allergies and autoimmune diseases. It has also become a standard tool in the …

The EliSpot (Enzyme Linked Immuno Spot) assay is designed to detect cell-mediated immune reactions to stimuli such as antigens to characterize the immune status and is based on the detection of …

Apr 17, 2025 · Learn how the ELISpot assay works, its applications in immunology and infectious disease research, and how to overcome common challenges in assay optimization.